5VF6
Crystal structure of single chain variable fragment (scFv45).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2016-05-24 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 97.232, 97.232, 61.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.103 - 1.635 |
| R-factor | 0.1735 |
| Rwork | 0.172 |
| R-free | 0.19630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p49 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.810 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.103 | 1.690 |
| High resolution limit [Å] | 1.635 | 1.640 |
| Rmerge | 0.049 | 0.442 |
| Number of reflections | 37409 | |
| <I/σ(I)> | 17.33 | 2.56 |
| Completeness [%] | 89.9 | 83.52 |
| Redundancy | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1M CAPS pH 10.0, 0.1M Ammonium Chloride, 40% PEG 400 |
