5VF2
scFv 2D10 re-refined as a complex with trehalose replacing the original alpha-1,6-mannobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 173.15 |
| Detector technology | CCD |
| Collection date | 2014-02-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.093, 81.093, 74.388 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.110 - 1.550 |
| R-factor | 0.15561 |
| Rwork | 0.153 |
| R-free | 0.18039 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5i4f |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.714 |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.549 |
| Number of reflections | 41534 |
| <I/σ(I)> | 39.67 |
| Completeness [%] | 99.4 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | MES 6.5, Magnesium sulphate (1.5 M), potassium sodium tartrate tetrahydrate (0.1M). |
