5VEQ

MOUSE KYNURENINE AMINOTRANSFERASE III, RE-REFINEMENT OF THE PDB STRUCTURE 3E2Y

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0809
Spacegroup name	P 43 21 2
Unit cell lengths	91.567, 91.567, 232.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.580 - 2.260
R-factor	0.1727
Rwork	0.170
R-free	0.22120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.736
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	29.580
High resolution limit [Å]	2.260
Number of reflections	42764
<I/σ(I)>	12.6
Completeness [%]	95.4
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	21% PEG 400, 150 MM CACL2, 10%, GLYCEROL, 100 MM HEPES, PH 7.5