5VEN

Murine ectonucleotide pyrophosphatase / phosphodiesterase 5 (ENPP5, NPP5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-17
Detector	RAYONIX MX-300
Wavelength(s)	0.9801
Spacegroup name	P 32 2 1
Unit cell lengths	82.403, 82.403, 301.010
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.202 - 1.690
R-factor	0.1304
Rwork	0.129
R-free	0.16400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.133
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.690
Number of reflections	133947
<I/σ(I)>	25.7
Completeness [%]	100.0
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	sodium acetate, PEG 200