5VE8

Crystal structure of full-length Kluyveromyces lactis Kap123 with histone H3 1-28

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	105
Detector technology	CCD
Collection date	2015-11-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	P 1
Unit cell lengths	79.098, 88.114, 102.276
Unit cell angles	79.59, 80.77, 70.74

Refinement procedure

Resolution	44.614 - 2.700
R-factor	0.1863
Rwork	0.185
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vch
RMSD bond length	0.008
RMSD bond angle	1.016
Data scaling software	HKL-2000 (HKL2000_v714-Linux)
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.043	0.587
Rpim	0.042	0.425
Number of reflections	69157	3411
<I/σ(I)>	18.8	1.56
Completeness [%]	99.0	98.3
Redundancy	4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296.15	0.1M sodium cacodylate (pH 6.5), 0.2M sodium acetate, 30% PEG 4000 and 5% Jeffamin M-600