5VCR

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase with bound uranium dioxide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-13
Detector	RAYONIX MX300-HS
Wavelength(s)	1.74
Spacegroup name	P 43 21 2
Unit cell lengths	105.670, 105.670, 171.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.397 - 1.992
R-factor	0.1782
Rwork	0.177
R-free	0.20800
RMSD bond length	0.017
RMSD bond angle	1.456
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.397
High resolution limit [Å]	1.992	1.992
Number of reflections	126205
<I/σ(I)>	17.7
Completeness [%]	99.0
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.4 M ammonium sulfate