Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.743, 73.263, 200.340 |
| Unit cell angles | 90.00, 91.09, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f5v 3rvt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.247 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.165 | 0.653 |
| Number of reflections | 28001 | |
| <I/σ(I)> | 10 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 MM MES, 10% W/V PEG 6000, 5% MPD, PH 7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
