5VCH
Crystal structure of full-length Kluyveromyces lactis Kap123
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2015-07-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.050, 88.120, 102.010 |
| Unit cell angles | 79.19, 80.03, 70.98 |
Refinement procedure
| Resolution | 33.001 - 2.350 |
| R-factor | 0.2104 |
| Rwork | 0.210 |
| R-free | 0.23490 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE (SHELXC/D/E) |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.520 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.200 | 0.615 |
| Rpim | 0.056 | 0.358 |
| Number of reflections | 105309 | 7667 |
| <I/σ(I)> | 21.3 | 2.5 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 12.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296.15 | 0.1M sodium cacodylate (pH 6.5), 0.2M sodium acetate, 30% PEG 4000 and 5% Jeffamin M-600 |
