5VBS
Structural basis for a six letter alphabet including GATCKX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-06 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 54.999, 145.643, 46.876 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.722 - 1.749 |
R-factor | 0.21 |
Rwork | 0.209 |
R-free | 0.23850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xo0 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.114 |
Data reduction software | DENZO |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.780 |
High resolution limit [Å] | 1.740 | 4.750 | 1.750 |
Rmerge | 0.038 | 0.027 | 0.299 |
Rmeas | 0.042 | 0.030 | 0.338 |
Rpim | 0.017 | 0.013 | 0.154 |
Number of reflections | 38374 | ||
<I/σ(I)> | 15.1 | ||
Completeness [%] | 98.4 | 95.3 | 92.3 |
Redundancy | 5.3 | 5 | 4.4 |
CC(1/2) | 0.998 | 0.932 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 10 % PEG 4000, 5 mM magnesium acetate and 50 mM ADA (pH 6.5) |