5VBK
Crystal structure of a galactose-binding Lectin from Mytilus californianus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 101 |
| Detector technology | PIXEL |
| Collection date | 2016-12-01 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.438, 63.690, 66.287 |
| Unit cell angles | 90.00, 120.49, 90.00 |
Refinement procedure
| Resolution | 35.088 - 1.788 |
| R-factor | 0.1851 |
| Rwork | 0.183 |
| R-free | 0.22490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wmv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.048 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((dev_2722)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.780 |
| Rmerge | 0.060 |
| Rpim | 0.040 |
| Number of reflections | 26332 |
| <I/σ(I)> | 28.8 |
| Completeness [%] | 95.1 |
| Redundancy | 4.4 |
| CC(1/2) | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M potassium thiocyanate, 0.1 M Bis-Tris Propane pH 8.5, 20% PEG 3350 |
