5VB8

Crystal structure of the NavAb voltage-gated sodium channel in an open state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	I 4 2 2
Unit cell lengths	125.302, 125.302, 192.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.530 - 2.850
R-factor	0.2295
Rwork	0.228
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rvy
RMSD bond length	0.013
RMSD bond angle	1.377
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.530	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.097
Number of reflections	16684
<I/σ(I)>	17
Completeness [%]	91.1
Redundancy	7.2
CC(1/2)		0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	277.15	1.8 M ammonium sulfate and 100 mM sodium acetate (pH 4.8). 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC):CHAPSO bicelles (Anatrace)