5VAU
Bcl-2 complex with Beclin 1 BH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-09 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.498, 53.337, 91.664 |
| Unit cell angles | 90.00, 107.84, 90.00 |
Refinement procedure
| Resolution | 34.323 - 1.754 |
| R-factor | 0.1882 |
| Rwork | 0.187 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xa0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.983 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.754 |
| Number of reflections | 78954 |
| <I/σ(I)> | 17.17 |
| Completeness [%] | 99.8 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M Bis-tris chloride, pH 5.5, ).2M ammonium acetate, 25% PEG 3350 |
