5VAO

Crystal structure of eVP30 C-terminus and eNP peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792601
Spacegroup name	I 41 2 2
Unit cell lengths	139.478, 139.478, 161.467
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	105.550 - 2.560
R-factor	0.1851
Rwork	0.183
R-free	0.22500
RMSD bond length	0.008
RMSD bond angle	1.209
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	105.550	50.000	2.590
High resolution limit [Å]	2.550	6.920	2.550
Rmerge	0.109	0.053	0.982
Rmeas	0.112	0.057
Rpim	0.042	0.021	0.497
Number of reflections	26327
<I/σ(I)>	6.2
Completeness [%]	99.7	100	94.9
Redundancy	7.6	7.3	4.3
CC(1/2)		0.990	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	297	0.2 M Calcium chloride dihydrate, 20%w/v PEG3350