5VAK

Crystal Structure of Beta-Klotho, Domain 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-12
Detector	RAYONIX MX325HE
Wavelength(s)	1.1808
Spacegroup name	C 1 2 1
Unit cell lengths	229.427, 49.346, 54.306
Unit cell angles	90.00, 100.22, 90.00

Refinement procedure

Resolution	48.208 - 1.700
R-factor	0.1729
Rwork	0.172
R-free	0.19640
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.208	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.054	0.440
Number of reflections	65245	5637
<I/σ(I)>	20.36	2.87
Completeness [%]	98.0	87
Redundancy	4.2	3.4
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	293	30% PEG 1000