5VAF

Crystal structure of accessory secretion protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2013-06-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9789
Spacegroup name	P 1
Unit cell lengths	41.502, 99.897, 179.069
Unit cell angles	100.66, 90.07, 95.78

Refinement procedure

Resolution	97.654 - 2.771
R-factor	0.212
Rwork	0.208
R-free	0.28160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vae
RMSD bond length	0.009
RMSD bond angle	1.069
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	97.654
High resolution limit [Å]	2.770
Rmerge	0.166
Number of reflections	66446
<I/σ(I)>	13.63
Completeness [%]	92.7
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	100 mM Bis-Tris Propane pH 9.2 28% PEG6000