5VAB

Crystal structure of ATXR5 PHD domain in complex with histone H3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-01-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.13
Spacegroup name	P 43 2 2
Unit cell lengths	44.010, 44.010, 71.948
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.536 - 1.702
R-factor	0.1504
Rwork	0.149
R-free	0.17860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qf3
RMSD bond length	0.005
RMSD bond angle	1.020
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.700
Number of reflections	8225
<I/σ(I)>	71.92
Completeness [%]	99.5
Redundancy	25.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1M Hepes (pH 6.5), 10% PEG 6000 and 5% (+/-)-2-methyl-2,4-pentanediol. SET : 50% polypropylene glycol 400, 5% DMSO, 0.1 M HEPES-NaOH (pH 6.0)