5V7X
Crystal Structure of Myosin 1b residues 1-728 with bound sulfate and Calmodulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.765, 48.730, 118.512 |
| Unit cell angles | 90.00, 89.95, 90.00 |
Refinement procedure
| Resolution | 39.504 - 3.141 |
| R-factor | 0.1928 |
| Rwork | 0.188 |
| R-free | 0.20230 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.834 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.504 |
| High resolution limit [Å] | 2.799 |
| Number of reflections | 30781 |
| <I/σ(I)> | 9.5 |
| Completeness [%] | 95.8 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 10% PEG 3350, 100 mM LiOOCCH3, 100 mM HEPES, pH 7.0, 20 mM Mg2SO4 |
