5V7E
T4 lysozyme Y18Ymcl
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-10-15 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.610, 59.610, 95.120 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.980 - 1.360 |
R-factor | 0.1751 |
Rwork | 0.174 |
R-free | 0.20530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l63 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.799 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.980 |
High resolution limit [Å] | 1.360 |
Number of reflections | 42545 |
<I/σ(I)> | 23.87 |
Completeness [%] | 99.7 |
Redundancy | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291 | 2.4 M Potassium phosphate, 50 mM 2-hydroxyethyl disulfide, 50 mM 2-mercaptoethanol |