5V7E
T4 lysozyme Y18Ymcl
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2016-10-15 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.610, 59.610, 95.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.980 - 1.360 |
| R-factor | 0.1751 |
| Rwork | 0.174 |
| R-free | 0.20530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.799 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.980 |
| High resolution limit [Å] | 1.360 |
| Number of reflections | 42545 |
| <I/σ(I)> | 23.87 |
| Completeness [%] | 99.7 |
| Redundancy | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291 | 2.4 M Potassium phosphate, 50 mM 2-hydroxyethyl disulfide, 50 mM 2-mercaptoethanol |
