5V74
Structure of the intact Haliangium ochraceum microcompartment shell
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03317 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 394.340, 638.090, 642.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.850 - 3.510 |
| R-factor | 0.280056559999 |
| Rwork | 0.279 |
| R-free | 0.32349 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EM map |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.864 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_2650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.690 | 3.690 |
| High resolution limit [Å] | 3.510 | 3.510 |
| Rmerge | 0.013 | 0.116 |
| Number of reflections | 880323 | 135274 |
| <I/σ(I)> | 3.6 | 0.4 |
| Completeness [%] | 98.2 | 92.7 |
| Redundancy | 15.8 | 13.9 |
| CC(1/2) | 0.260 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 6% PEG-20000 0.1M Bicine pH 8.9 |
