5V6R

Structure of Plexin D1 intracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	69.852, 164.544, 84.268
Unit cell angles	90.00, 99.47, 90.00

Refinement procedure

Resolution	37.095 - 2.700
R-factor	0.2165
Rwork	0.215
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ig3
RMSD bond length	0.003
RMSD bond angle	0.616
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Rpim	0.042	0.231
Number of reflections	51129
<I/σ(I)>	29.9
Completeness [%]	99.4
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	0.1 M imidazole (pH 7.8) and 15% (w/v) PEG8000