5V6K
Crystal Structure of the Second beta-Prism Domain of RbmC from V. cholerae Bound to N-acetylglucosaminyl-beta-1,2-mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-01-17 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.940, 38.770, 51.890 |
| Unit cell angles | 90.00, 105.26, 90.00 |
Refinement procedure
| Resolution | 30.652 - 1.800 |
| R-factor | 0.1667 |
| Rwork | 0.166 |
| R-free | 0.18930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xez |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.586 |
| Data reduction software | MOSFLM (7.2.1) |
| Data scaling software | Aimless (0.5.31) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.770 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.077 | 0.477 |
| Rmeas | 0.089 | 0.608 |
| Rpim | 0.044 | 0.370 |
| Total number of observations | 41463 | 805 |
| Number of reflections | 10585 | 321 |
| <I/σ(I)> | 11.4 | 1.8 |
| Completeness [%] | 91.9 | 47.2 |
| Redundancy | 3.9 | 2.5 |
| CC(1/2) | 0.996 | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1 M sodium acetate, 2 M sodium sulfate, 1 mM N-acetylglucosaminyl-beta-1,2-mannose |
