5V6J

Glycan binding protein Y3 from mushroom Coprinus comatus possesses anti-leukemic activity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 1 21 1
Unit cell lengths	53.265, 56.098, 62.728
Unit cell angles	90.00, 92.69, 90.00

Refinement procedure

Resolution	41.795 - 1.180
Rwork	0.167
R-free	0.18200
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.795	1.220
High resolution limit [Å]	1.179	1.180
Rmerge	0.041	0.298
Number of reflections	113409
<I/σ(I)>	22.98	4.4
Completeness [%]	90.0	80
Redundancy	5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	298	16% PEG4000, 10% V/V GLYCEROL, 0.1 M CHES, PH 9.5