5V6G

Crystal structure of Influenza A virus Matrix Protein M1(NLS-88R)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-11-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	39.746, 119.818, 59.684
Unit cell angles	90.00, 90.20, 90.00

Refinement procedure

Resolution	29.840 - 2.000
R-factor	0.192
Rwork	0.192
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aa7
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.950	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.088	0.278
Number of reflections	33892	3432
<I/σ(I)>	13.1	5.9
Completeness [%]	90.0	91.9
Redundancy	6.05	5.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	Protein solution: 13.7 mg/ml in 55 mM K2HPO4/KH2PO4/H3PO4, 0.2M NaCl, 10 mM BME, pH 4.0 Reservoir solution: 75 mM Tris-HCl, pH 7.0, 15% PEG-1000