5V69

Crystal structure of the Middle East respiratory syndrome coronavirus papain-like protease bound to ubiquitin variant ME.4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2014-07-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5419
Spacegroup name	C 2 2 21
Unit cell lengths	48.207, 110.965, 185.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.640 - 2.550
R-factor	0.2021
Rwork	0.197
R-free	0.24550
RMSD bond length	0.002
RMSD bond angle	0.467
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.640
High resolution limit [Å]	2.550
Number of reflections	16565
<I/σ(I)>	10.9
Completeness [%]	99.0
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	294.15	0.1 M trisodium citrate pH 5.6, 20% (w/v) polyethylene glycol (PEG) 4000, 20% (v/v) isopropanol