5V4P

Saccharomyces cerevisiae OYE 3 soaked with p-hydroxybenzaldehyde

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	61.533, 106.421, 141.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.924 - 1.880
R-factor	0.1838
Rwork	0.182
R-free	0.21820
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	24.924
High resolution limit [Å]	1.880
Number of reflections	76003
<I/σ(I)>	18.61
Completeness [%]	100.0
Redundancy	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	100 mM sodium citrate tribasic dihydrate, 30% (v/v) polyethylene glycol monomethyl ether 550