5V47
Crystal structure of the SR1 domain of lizard sacsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.977 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.779, 38.164, 114.721 |
| Unit cell angles | 90.00, 131.55, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.840 |
| R-factor | 0.19732 |
| Rwork | 0.195 |
| R-free | 0.23401 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5v45 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.275 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 43261 | 2962 |
| <I/σ(I)> | 20.4 | 2.9 |
| Completeness [%] | 99.3 | 91.76 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 0.2 M lithium sulfate, 25% PEG3350 |
