5V43

Engineered human IgG Fc domain aglyco801

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	P 65 2 2
Unit cell lengths	66.260, 66.260, 370.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.915 - 2.320
R-factor	0.1969
Rwork	0.195
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.550
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2142))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.360
High resolution limit [Å]	2.320	2.320
Number of reflections	22297	1090
<I/σ(I)>	25.625	2
Completeness [%]	100.0	100
Redundancy	9.2	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	50 mM sodium citrate pH 5.0 0.44 M NaCl 24% w/v PEG 4000