5V3J
mouseZFP568-ZnF1-10 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-21 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.787, 65.706, 73.022 |
| Unit cell angles | 100.52, 104.25, 97.25 |
Refinement procedure
| Resolution | 34.503 - 2.064 |
| R-factor | 0.1936 |
| Rwork | 0.192 |
| R-free | 0.23710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.049 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 2.130 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.076 | 0.764 |
| Rpim | 0.425 | 0.425 |
| Number of reflections | 58341 | 5723 |
| <I/σ(I)> | 12.9 | 1.9 |
| Completeness [%] | 97.9 | 96.6 |
| Redundancy | 4.4 | 4.3 |
| CC(1/2) | 0.941 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 8% Tacsimate(pH 5.0) and 20% (W/V) PEG3350 |
