5V3I

Crystal structure of the VS ribozyme - wild-type C634

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-31
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97921
Spacegroup name	P 43 21 2
Unit cell lengths	104.306, 104.306, 211.906
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.583 - 3.293
R-factor	0.219
Rwork	0.216
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r4v
RMSD bond length	0.003
RMSD bond angle	0.699
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.621	3.410
High resolution limit [Å]	3.290	3.290
Rmerge	0.115
Number of reflections	18250	18250
<I/σ(I)>	14.08
Completeness [%]	98.9	98.89
Redundancy	9.8
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	0.1 M sodium cacodylate (pH 6.8), 1.95 M ammonium sulfate