5V3G
PRDM9-allele-C ZnF8-13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-22 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.151, 123.778, 70.200 |
| Unit cell angles | 90.00, 116.07, 90.00 |
Refinement procedure
| Resolution | 35.183 - 2.416 |
| R-factor | 0.1758 |
| Rwork | 0.173 |
| R-free | 0.22540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.267 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.200 | 2.520 |
| High resolution limit [Å] | 2.416 | 2.420 |
| Rmerge | 0.076 | 0.721 |
| Rpim | 0.031 | 0.442 |
| Number of reflections | 35662 | 2949 |
| <I/σ(I)> | 22.68 | 1.3 |
| Completeness [%] | 96.5 | 72.3 |
| Redundancy | 6.5 | 2.9 |
| CC(1/2) | 0.541 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 289 | 0.1 M Bis-Tris Propane (pH-7.3) and 23% W/V PEG3350 |
