5V1I

DNA polymerase beta ternary product complex with 8-oxoG:C and inserted dCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-21
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	50.564, 79.798, 55.539
Unit cell angles	90.00, 107.62, 90.00

Refinement procedure

Resolution	24.596 - 2.035
R-factor	0.188
Rwork	0.184
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fms
RMSD bond length	0.007
RMSD bond angle	0.892
Data scaling software	HKL-3000
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.035
Number of reflections	47251
<I/σ(I)>	14.4
Completeness [%]	98.3
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	50 mM imidazole, 350 mM sodium chloride, 17% PEG3350