5V13
Mosquito juvenile hormone-binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979010 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.150, 69.070, 121.940 |
| Unit cell angles | 90.00, 112.81, 90.00 |
Refinement procedure
| Resolution | 55.376 - 1.840 |
| R-factor | 0.1655 |
| Rwork | 0.164 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.828 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.6.1) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | |
| Low resolution limit [Å] | 55.376 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.102 | 0.525 |
| Number of reflections | 78904 | |
| <I/σ(I)> | 10.8 | 2.73 |
| Completeness [%] | 98.5 | 99.8 |
| Redundancy | 3.5 | 3.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1 M MES, pH 6, 30% PEG 6000, 3.5 % sorbitol |
