5V0O
RNA hairpin structure containing 2-MeImpG analogue bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2016-12-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 44.747, 44.747, 154.198 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.970 - 2.700 |
R-factor | 0.2205 |
Rwork | 0.218 |
R-free | 0.27661 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fnj |
RMSD bond length | 0.012 |
RMSD bond angle | 1.781 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.100 | 0.777 |
Number of reflections | 4657 | 437 |
<I/σ(I)> | 18.24 | 1.65 |
Completeness [%] | 97.3 | 94.4 |
Redundancy | 9.1 | 6.4 |
CC(1/2) | 0.984 | 0.931 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 617-726-5102 |