5V0K
RNA duplex with 2-MeImpG analogue bound-3 binding sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2015-10-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 42.229, 42.229, 40.098 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.100 - 1.600 |
| R-factor | 0.17668 |
| Rwork | 0.174 |
| R-free | 0.22601 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hbx |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.937 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.083 | 0.364 |
| Number of reflections | 5712 | 536 |
| <I/σ(I)> | 23.54 | 2.41 |
| Completeness [%] | 99.5 | 94.9 |
| Redundancy | 8.9 | 4.1 |
| CC(1/2) | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.04 M Lithium chloride, 0.08 M Strontium chloride hexahydrate 0.04 M Sodium cacodylate trihydrate pH 7.0 30% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride, 0.02 M Magnesium chloride |
