5V0I
Crystal Structure of Tryptophanyl-tRNA Synthetase from Escherichia coli Complexed with AMP and Tryptophan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.745, 110.807, 128.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.933 - 1.900 |
| R-factor | 0.1872 |
| Rwork | 0.186 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n9i |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.931 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 52495 | 1883 |
| <I/σ(I)> | 15.8 | 1.146 |
| Completeness [%] | 97.4 | 70.5 |
| Redundancy | 6.6 | 3.9 |
| CC(1/2) | 0.828 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 15% PEG 1500 |
