5V0E
Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' flap DNA (f5I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.776, 72.776, 181.201 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.458 - 2.744 |
| R-factor | 0.2073 |
| Rwork | 0.204 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qea |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.532 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.910 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.060 | 0.571 |
| Rmeas | 0.067 | 0.635 |
| Number of reflections | 24257 | 3874 |
| <I/σ(I)> | 16.19 | 2.33 |
| Completeness [%] | 99.5 | 97.6 |
| Redundancy | 5.14 | 5.19 |
| CC(1/2) | 0.999 | 0.887 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000 |
