5V0C
Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' flap DNA (f2I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.863, 71.863, 181.825 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.331 - 2.580 |
| R-factor | 0.2325 |
| Rwork | 0.229 |
| R-free | 0.27250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qea |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.452 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.730 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.042 | 0.565 |
| Rmeas | 0.049 | 0.662 |
| Number of reflections | 28650 | 4605 |
| <I/σ(I)> | 19.82 | 2.3 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 3.74 | 3.74 |
| CC(1/2) | 0.877 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000 |
