5V06

Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' recessed-end DNA (rIV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	74.281, 74.281, 179.889
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.471 - 2.750
R-factor	0.2224
Rwork	0.218
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qea
RMSD bond length	0.002
RMSD bond angle	0.458
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.750	2.750
Rmerge	0.092	0.695
Rmeas	0.101	0.756
Number of reflections	13868	671
<I/σ(I)>	19.19	2.56
Completeness [%]	99.2	100
Redundancy	6	6.5
CC(1/2)	0.698

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000