5V04

Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' recessed-end DNA (rII)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	73.815, 73.815, 180.947
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.570 - 2.650
R-factor	0.2314
Rwork	0.229
R-free	0.25580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qea
RMSD bond length	0.003
RMSD bond angle	0.535
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.081	0.655
Rmeas	0.086	0.708
Number of reflections	15277	707
<I/σ(I)>	26.65	1.82
Completeness [%]	99.6	98.5
Redundancy	8.9	6.6
CC(1/2)	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000