5UZV

Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' recessed-end DNA (rI)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	72.488, 72.488, 182.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.200 - 2.450
R-factor	0.2166
Rwork	0.213
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qea
RMSD bond length	0.002
RMSD bond angle	0.482
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.059	0.656
Rmeas	0.064	0.716
Number of reflections	18545	907
<I/σ(I)>	32.69	2.36
Completeness [%]	98.9	100
Redundancy	6.3	6.4
CC(1/2)	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000