5UZP
Crystal structure of citrate synthase mutant A348G from homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.245, 111.804, 74.162 |
| Unit cell angles | 90.00, 99.31, 90.00 |
Refinement procedure
| Resolution | 45.060 - 2.290 |
| R-factor | 0.15824 |
| Rwork | 0.156 |
| R-free | 0.20549 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.606 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.129 | 0.768 |
| Rmeas | 0.176 | 1.194 |
| Rpim | 0.091 | 0.618 |
| Number of reflections | 40046 | 2413 |
| <I/σ(I)> | 10.8 | 1.75 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M Ammonium Acetate, 0.1 NaCitrate tribasic dihydrate pH 5.6, 15% PEG4000 |
