5UZ8
Crystal Structure of Mouse Cadherin-23 EC22-24
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.537, 57.208, 114.667 |
Unit cell angles | 90.00, 112.55, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.850 |
R-factor | 0.17136 |
Rwork | 0.171 |
R-free | 0.18788 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5i8d |
RMSD bond length | 0.011 |
RMSD bond angle | 1.496 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.087 | 0.657 |
Number of reflections | 45949 | 2224 |
<I/σ(I)> | 12.8 | 1.75 |
Completeness [%] | 99.5 | 97.9 |
Redundancy | 3.3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M HEPES pH 7.5 3.5 M Ammonium Chloride |