5UYZ

Structure of Human T-complex protein 1 subunit epsilon (CCT5) mutant His147Arg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 4 21 2
Unit cell lengths	205.570, 205.570, 163.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.417 - 3.600
R-factor	0.2773
Rwork	0.275
R-free	0.32410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uyx
RMSD bond length	0.004
RMSD bond angle	0.896
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((dev_2650: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	67.000
High resolution limit [Å]	3.600
Number of reflections	41130
<I/σ(I)>	10.9
Completeness [%]	100.0
Redundancy	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 MSodium acetate, 0.04 M citric acid, 0.06 M Bis-Tris propane pH 6.4, and 25% PEG 400