5UYX
Structure of Human T-complex protein 1 subunit epsilon (CCT5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 204.710, 204.710, 162.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.700 - 3.500 |
| R-factor | 0.2711 |
| Rwork | 0.269 |
| R-free | 0.31820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v81 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.663 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2650: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 75.700 |
| High resolution limit [Å] | 3.500 |
| Number of reflections | 44068 |
| <I/σ(I)> | 13.7 |
| Completeness [%] | 100.0 |
| Redundancy | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 298 | 0.1 M Sodium acetate, 0.04 Mcitric acid, 0.06 MBis-tris propane pH 6.4, 25 %PEG400 |
