5UYT
Crystal structure of ice binding protein from an Antarctic bacterium Flavobacteriaceae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 119.826, 119.826, 367.764 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.080 - 1.750 |
| R-factor | 0.16892 |
| Rwork | 0.168 |
| R-free | 0.19051 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.457 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.080 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 101962 | 10103 |
| <I/σ(I)> | 29.1 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.6 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 15-20% PEG 3350, 0.2 M ammonium nitrate |
