5UXS
X ray structure of the periplasmic ligand binding protein YfeA from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-26 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.28281 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.736, 52.120, 113.066 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.577 - 1.421 |
| R-factor | 0.1793 |
| Rwork | 0.179 |
| R-free | 0.19030 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.760 |
| Data reduction software | HKL-2000 (v712) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.098 | 0.401 |
| Rpim | 0.051 | 0.213 |
| Number of reflections | 47252 | 2340 |
| <I/σ(I)> | 52.8 | 2.2 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 4.6 | 4.5 |
| CC(1/2) | 0.981 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.3 | 293.15 | 30-35% PEG 4000, 50mM sodium chloride, 20mM Bis-tris propane, 0.05% w/v sodium azide |
