5UXG
Protein 84 with aldehyde deformylating oxygenase activity from Sulfolobus tokodaii (monoclinic)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.536, 64.849, 71.299 |
| Unit cell angles | 90.00, 116.86, 90.00 |
Refinement procedure
| Resolution | 63.610 - 1.720 |
| R-factor | 0.1961 |
| Rwork | 0.195 |
| R-free | 0.22341 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | theoretical |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.760 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.610 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.050 | 0.251 |
| Number of reflections | 55207 | |
| <I/σ(I)> | 20.2 | 3.7 |
| Completeness [%] | 96.5 | 57 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Potassium phosphate, PEG 8000 |
