5UX9
The crystal structure of chloramphenicol acetyltransferase from Vibrio fischeri ES114
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 63 |
| Unit cell lengths | 147.426, 147.426, 101.211 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.045 - 2.700 |
| R-factor | 0.2018 |
| Rwork | 0.200 |
| R-free | 0.23970 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.568 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.055 | 0.779 |
| Rpim | 0.027 | 0.400 |
| Number of reflections | 34158 | 1724 |
| <I/σ(I)> | 21.6 | 2.5 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 4.2 | 4.1 |
| CC(1/2) | 0.705 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M Magnesium Chloride, 0.1 M MES:NaOH, 10% (w/v) PEG4000 |
