5UX6
Structure of Human POFUT1 in its apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.198, 132.236, 72.055 |
| Unit cell angles | 90.00, 106.44, 90.00 |
Refinement procedure
| Resolution | 47.780 - 2.090 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zy4 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.710 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.087 | 2.090 |
| Number of reflections | 52549 | |
| <I/σ(I)> | 7.42 | |
| Completeness [%] | 98.2 | 97.6 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 16.5% v/v PEG3350, 0.05% w/v octyl-beta-D-glucoside, 0.5% v/v glycerol, and 100 mM sodium citrate pH 5.0 |
