5UX3
RNA hairpin structure containing 2-MeImpG monomer analogue and 2-MeImp-oligomer analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2016-10-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 46.527, 46.527, 156.938 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.610 - 2.500 |
R-factor | 0.2204 |
Rwork | 0.218 |
R-free | 0.26127 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fnj |
RMSD bond length | 0.015 |
RMSD bond angle | 2.564 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (v3.0) |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.080 | 0.695 |
Number of reflections | 6436 | 604 |
<I/σ(I)> | 20.3 | 1 |
Completeness [%] | 98.7 | 94.1 |
Redundancy | 5.1 | 3.9 |
CC(1/2) | 0.943 | 0.754 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 50 mM MgCl2, 3 M ammonia sulfate, 100 mM HEPES 7.5 |